Biodegradation and Decolorization of Reactive Dye Red ME4BL by Bacillus subtilis

A bacterial strain SVM1 with remarkable ability to decolorization textile dye Reactive Red ME4BL was isolated from the activated sludge soil collected from a common effluent treatment plant (CETP) in Perunduari, Erode district Tamil Nadu. Different parameters such as temperature and pH were optimized for decolorization of Red ME4BL by using isolated bacteria. The most promising bacterial isolated was used to further dye degradation studies. The 16 s RNA gene sequencing isolated organisms as Bacillus subtilis strain SVM1 the strain showed 93% decolorization of the selected dye Reactive Red ME4BL 200mg/1000ml within 42 hours in static and shaking condition. The optimum pH and temperature for the decolorization was 8 and 37°C respectively. The biodegradation was analysis by UV-Vis, FTIR and HPLC. The result suggested that the isolated organisms Bacillus subtilis strain SVM1 can be used useful tool to treat waste water containing textile reactive dye.